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- Claude Bernard -
“The joy of discovery is certainly the liveliest that the mind of man can ever feel”
SYNTHESIS NANOPARTICLES
1. Synthesis Quantum dots
The synthesis quantum dot was based on the following four-step colloidal synthesis procedure: (1) synthesis of core host particles (CdSSe), (2) Mn2+-dopant growth, (3) host-shell growth, and (4) ligand exchange. In the first step, CdSSe (a CdS core (bulk CdS band gap ≈ 2.42 eV) doped with CdSe (bulk CdSe band gap ≈ 1.74 eV)) was used as a core because its excitonic emission can be brought closer to the Mn2+-excited state, allowing efficient coupling 58. ZnS was selected as the shell material because of the availability of well-developed Qdot passivation techniques using water-soluble ligands 60,73,79. The core used in this experiment was CdSSe with a diameter of 3.1 nm. After the growth of the Mn2+-dopant and ZnS shell, the diameter of the Mn2+-doped CdSSe/ZnS (i.e., the MnQdots) was 8.3 nm. After obtaining the MnQdots , on the surfaces of MnQdots were decorated with includingD-penicillamine (DPA), pH sensitive ligands.
2. Synthesis FORMSN
The synthesis triple labeled MSN have been conducted by co-condensation of two pH-sensitives dyes APTMS conjugated-(FITC/OG) and one reference dye, APTMS conjugated RITC, with cationic surfactant CTAB and TEOS in ammonium hydroxide solution. In this experiment, ammonium hydroxide solution (1.024M)have been used to get the FORMSN with diameter 200 nm . For improving the uniformity of the triple-labeled MSNs, the additional ethylene glycol (EG) was added in the overall process of the synthesis. During the process of silicate condensation, the EG would interact with the surface of the forming silica structure based on a template of cationic surfactants. The interaction could regulate the size of the forming particles and result in good uniformity of the triple-labeled MSNs . The triple-labeled MSNs were also modified with polyethylene glycol (PEG) and PEI (Poly on the surface for achieving the mono-dispersion in medium its name FORMSN (+).
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